GenScript presents a range of ready-to-use vectors tailored for mRNA researchers that enhance and surpass the conventional pUC57 and pVAX1 vectors by offering heightened cloning efficiency, poly(A) tail stability, and plasmid yield. Our offerings encompass prelinearized vectors complete with key components necessary for in vitro transcription. These vectors are available in two distinctive backbones, each coupled with four different poly(A) sequence variations and a duo of restriction enzyme digestion sites for tailored applications.
More than 2-fold cloning efficiency compare to traditional pUC57 vectors
Superior Poly(A)
Stability
One-Stop Solution
with our Gene Synthesis, Plasmid Prep, and Cloning Services
GS-mK Vector
Description:
The GS-MK Vector has been adapted using the pUC57-kana Vector. This involved removing the Lac promoter sequence, and placing terminator elements both before and after the MCS. These modifications have improved poly(A) tail stability and cloning efficiency.
| Plasmid Type | Cloning Vector |
| Derived from | pUC57-kana |
| Promoter | N/A |
| Resistance | Kanamycin |
| Cloning Method | Restriction Enzyme/MCS |
| Poly(A) | Empty/100A/120A/30+30+43A/31+71A |
| Cleavage Site | BspQI/BsaI |
GS-CMV Vector
Description:
The GS-CMV Vector has been adapted using the pVAX1 Vector, a distinctive vector approved by the US FDA for clinical trials and suitable for eukaryotic transfection expression assays. GS-CMV has also incorporated terminator elements to enhance the stability of the poly(A) tail and has amplified its plasmid copy number. This ensures a decrease in potential genome contamination and minimizes the loss of quantity during plasmid extraction.
| Plasmid Type | Expression Vector |
| Derived from | pVAX1 |
| Promoter | CMV |
| Resistance | Kanamycin |
| Cloning Method | Restriction Enzyme/MCS |
| Poly(A) | Empty/100A/120A/30+30+43A/31+71A |
| Cleavage Site | BspQI/BsaI |
*Our mRNA vector has been deliberately crafted without a promoter. For more information please refer to the FAQ
Figure 1. Gene Fragment encompassing a polyadenylate tract of 100 adenosine residues was independently cloned into pUC57-kana and GS-mK vectors. These recombinant constructs were subsequently transformed into a competent GenPoly bacterial strain for propagation. Isolated clones were subjected to molecular screening and sequencing to verify the integrity of the gene fragments. Clonal efficiency was quantified by assessing the proportion of clones harboring an intact insert.
Figure 1. The plasmid production capability was evaluated by comparing the pVAX1 vector with the GS-CMV vector. Following the transformation of the respective vectors into the competent GenPoly strain, single colonies were selected and grown in 400 mL of 3xLB medium. The cultures were then incubated at 37°C for 13 hours to promote the growth of the bacterial culture. Post incubation, the plasmids were extracted, and the graph illustrates the total plasmid yield obtained.
1. Why does your mRNA vector lack a T7 Promoter?
A: Our mRNA vector has been deliberately crafted without a promoter and UTR regions to allow users the versatility to customize their experiments with their selected promoter and UTR sequences. To adapt the vector to your needs, just integrate your chosen promoter and UTRs alongside your ORF during the cloning process into the MCS region.
2. Can I license the mRNA applied vector for commercial use?
Certainly, we provide a commercial licensing service for clients intending to utilize our mRNA applied vectors for commercial purposes. Please contact us for further information.
3. Can I request more information on the vectors?
Certainly, we have extensive data in regards to our vector, such as Poly(A) tail stability, in vitro transcription (IVT) mRNA purity, and IVT mRNA expression levels. For more detailed information, please get in touch with us.
Tailor and authenticate the polyadenylation of your mRNA template
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Ideal for scientists seeking high-quality large-scale mRNA IVT template
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Basic research to animal study grade
Plasmid DNA from microgram to gram level
Poly(A) and AAV ITR guarantee
